This ability provides better understanding of the most relevant targets to investigate for their therapeutic potential in hypodiploid acute lymphoblastic leukemia.ĭiaz-Flores and his colleagues found elevated levels of both pro-survival and anti-survival proteins in leukemic cells. Diaz-Flores cites the resolution of the ChemiDoc Touch Imaging System for enabling him to visualize very faint bands and to distinguish and quantitate darker bands. Images created using ChemiDoc Touch Imaging System (left panel) and X-ray (right panel) are shown. This set of Ernesto Diaz-Flores’s western blot images shows Bcl-2 family proteins in different samples of leukemic cells. There are 12 to 15 members of this family.” “We have been focusing on the Bcl-2 family members, which are involved in the survival pathway. Mignon Loh’s lab at the University of California, San Francisco.
“As a follow-up to that study, I have been pursuing the protein pathways that we found were most deregulated,” says Ernesto Diaz-Flores, a researcher in Dr. Along with a detailed genomic profile, this study also indicated several protein pathway alterations that merited further investigation for possible therapeutic targets. In early 2013, Nature Genetics published a study by a group of researchers that provided the first broad characterization of hypodiploid acute lymphoblastic leukemia, a subtype of childhood leukemia that has proven highly resistant to treatment. In this video, Ernesto Diaz-Flores describes using the ChemiDoc Touch Imaging System to distinguish potential therapeutic targets in translational leukemia research, as well as how his interest in photography gives him a special perspective on digital imaging for protein analysis. Distinguishing the Best Targets for Novel Leukemia Treatments Each sat down for an interview regarding how he used the ChemiDoc Touch Imaging System and provided a representative blot image created using the instrument. Some reported having tried digital imaging in the past, but not being convinced at that time to give up film. Each one started the process as a user of X-ray film for western blot imaging. We gave this new imaging system to several researchers to test. As a part of Bio-Rad’s V3 Western Workflow, the ChemiDoc Touch Imaging System enables a western blotting protocol improved with multiple validation steps, streamlined completion times, and quantitative results using stain-free gel chemistry.
BIO RAD WESTERN BLOT BAND NUMBERS SOFTWARE
Designed for efficiency and ease of use, it integrates a touch-screen interface with Image Lab™ Touch Software to give users a fast workflow for acquiring and analyzing publication-quality image data. The ChemiDoc Touch Imaging System is Bio-Rad’s new digital imaging solution for gel and western blot analysis.
BIO RAD WESTERN BLOT BAND NUMBERS PDF
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